P1304MP - Propidium Iodide

Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population. PI binds to DNA by intercalating between the bases with little or no sequence preference. In aqueous solution, the dye has excitation/emission maxima of 493/636 nm. Once the dye is bound, its fluorescence is enhanced 20- to 30-fold, the fluorescence excitation maximum is shifted ~30–40 nm to the red and the fluorescence emission maximum is shifted ~15 nm to the blue, resulting in an excitation maximum at 535 nm and fluorescence emission maximum at 617 nm. PI is widely used in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry. Contents & storage Contains 1 vial propidium iodide (100 mg). Store at room temperature and protect from light.

12557013 - PB-MAX™ Karyotyping Medium

Gibco® PB-MAX™ Karyotyping Medium is a fully-supplemented medium developed for the short-term culture of peripheral blood lymphocytes for cytogenetic studies and in vitro diagnostic procedures. Gibco® PB-MAX™ Karyotyping Medium features: • Ready-to-use format • Quality and performance testing • Classic formulation Ready-to-use format Gibco® PB-MAX™ Karyotyping Medium is a frozen, 1X medium, ready to use upon thawing. No supplementation is required. Thawed medium can be stored at 2–8°C for up to 14 days. Quality and performance testing Every lot of Gibco® PB-MAX™ Karyotyping Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Peripheral blood lymphocytes from a normal adult donor are cultured for 72 hours in Gibco® PB-MAX™ Karyotyping Medium before measuring the mitotic index and chromosome banding resolution. In addition, each lot is tested for sterility, pH, osmolality, and mycoplasma. Classic formulation Gibco® PB-MAX™ Karyotyping Medium is an optimized RPMI 1640 medium, supplemented with Fetal Bovine Serum (FBS), L-glutamine, and phytohemagglutinin (PHA). Gibco® PB-MAX™ Karyotyping Medium is based on formulations referenced in the Association for Cytogenetic Technologists Laboratory Manual for the culture of peripheral blood lymphocytes for cytogenetic analysis. The complete formulation is available. Product Use For in vitro diagnostic use. cGMP Manufacturing and Quality System Gibco® PB-MAX™ Karyotyping Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. Contents & storage Storage conditions: -5 to -20°C. Protect from light. Shipping conditions: Frozen Shelf life: 12 months from date of manufacture

V22885 - Vybrant™ DiI Cell-Labeling Solution

DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes. This orange—red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a long-term tracer for neuronal and other cells. DiI is also available as a solid (D-282), as a paste (N-22880) or as crystals (D-3911). Contents & storage Store at room temperature and protect from light.

17001082 - AmnioMAX™ C-100 Basal Medium

Gibco® AmnioMAX™ C-100 Basal Medium is part of a kit used to prepare a fully-supplemented Gibco® AmnioMAX™ C-100 Complete Medium developed for the short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures. Gibco® AmnioMAX™ C-100 Complete Medium features: • Easy-to-use format • Quality and performance testing • Unique, optimized formulation Easy-to-use format Gibco® AmnioMAX™ C-100 Complete Medium is a two-part kit for preparation of a complete medium that requires no additional supplementation. The complete medium can be stored at 2–8°C for up to ten days. Quality and performance testing Every lot of Gibco® AmnioMAX™ C-100 Complete Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Pooled primary human amniotic fluid samples are cultured for six days in Gibco® AmnioMAX™ C-100 Complete Medium before measuring the total number of colonies and total number of mitotic colonies. In addition, each lot is tested for sterility, pH and osmolality. Unique, optimized formulation Gibco® AmnioMAX™ C-100 Complete Medium contains Fetal Bovine Serum (FBS), gentamicin, and L-glutamine to maximize cell attachment and growth. This optimized medium also has an enhanced buffering system that provides greater pH stability during culture manipulations. Product Use For in vitro diagnostic use. cGMP Manufacturing and Quality System Gibco® AmnioMAX™ C-100 Basal Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. Contents & storage Storage conditions: 2 – 8°C Shipping conditions: Ambient Shelf life: 16 months from date of manufacture. Use complete medium within 7–10 days once opened.

17001074 - AmnioMAX™ C-100 Basal Medium

Gibco® AmnioMAX™ C-100 Basal Medium is part of a kit used to prepare a fully-supplemented Gibco® AmnioMAX™ C-100 Complete Medium developed for the short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures. Gibco® AmnioMAX™ C-100 Complete Medium features: • Easy-to-use format • Quality and performance testing • Unique, optimized formulation Easy-to-use format Gibco® AmnioMAX™ C-100 Complete Medium is a two-part kit for preparation of a complete medium that requires no additional supplementation. The complete medium can be stored at 2–8°C for up to ten days. Quality and performance testing Every lot of Gibco® AmnioMAX™ C-100 Complete Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Pooled primary human amniotic fluid samples are cultured for six days in Gibco® AmnioMAX™ C-100 Complete Medium before measuring the total number of colonies and total number of mitotic colonies. In addition, each lot is tested for sterility, pH and osmolality. Unique, optimized formulation Gibco® AmnioMAX™ C-100 Complete Medium contains Fetal Bovine Serum (FBS), gentamicin, and L-glutamine to maximize cell attachment and growth. This optimized medium also has an enhanced buffering system that provides greater pH stability during culture manipulations. Product Use For in vitro diagnostic use. cGMP Manufacturing and Quality System Gibco® AmnioMAX™ C-100 Basal Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. Contents & storage Storage conditions: 2 – 8°C Shipping conditions: Ambient Shelf life: 16 months from date of manufacture. Use complete medium within 7–10 days once opened.

12556015 - AmnioMAX™ C-100 Supplement

Gibco® AmnioMAX™ C-100 Supplement is part of a kit used to prepare a fully-supplemented Gibco® AmnioMAX™ C-100 Complete Medium developed for the short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures. Gibco® AmnioMAX™ C-100 Complete Medium features: • Easy-to-use format • Quality and performance testing • Unique, optimized formulation Easy-to-use format Gibco® AmnioMAX™ C-100 Complete Medium is a two-part kit for preparation of a complete medium that requires no additional supplementation. The complete medium can be stored at 2–8°C for up to ten days. Quality and performance testing Every lot of Gibco® AmnioMAX™ C-100 Complete Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Pooled primary human amniotic fluid samples are cultured for six days in Gibco® AmnioMAX™ C-100 Complete Medium before measuring the total number of colonies and total number of mitotic colonies. In addition, each lot is tested for sterility, pH and osmolality. Unique, optimized formulation Gibco® AmnioMAX™ C-100 Complete Medium contains Fetal Bovine Serum (FBS), gentamicin, and L-glutamine to maximize cell attachment and growth. This optimized medium also has an enhanced buffering system that provides greater pH stability during culture manipulations. Product Use For in vitro diagnostic use. cGMP Manufacturing and Quality System Gibco® AmnioMAX™ C-100 Supplement is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. Contents & storage Storage conditions: -5 to -20°C Shipping conditions: Frozen Shelf life: 16 months from date of manufacture. Use complete medium within 7–10 days once opened.

12558011 - AmnioMAX™ C-100 Complete Medium

Gibco® AmnioMAX™ C-100 Complete Medium is a kit used to prepare a fully-supplemented medium developed for the short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures. Gibco® AmnioMAX™ C-100 Complete Medium features: • Easy-to-use format • Quality and performance testing • Unique, optimized formulation Easy-to-use format Gibco® AmnioMAX™ C-100 Complete Medium is a two-part kit for preparation of a complete medium that requires no additional supplementation. The complete medium can be stored at 2–8°C for up to ten days. Quality and performance testing Every lot of Gibco® AmnioMAX™ C-100 Complete Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Pooled primary human amniotic fluid samples are cultured for six days in Gibco® AmnioMAX™ C-100 Complete Medium before measuring the total number of colonies and total number of mitotic colonies. In addition, each lot is tested for sterility, pH and osmolality. Unique, optimized formulation Gibco® AmnioMAX™ C-100 Complete Medium contains Fetal Bovine Serum (FBS), gentamicin, and L-glutamine to maximize cell attachment and growth. This optimized medium also has an enhanced buffering system that provides greater pH stability during culture manipulations. Product Use For in vitro diagnostic use. cGMP Manufacturing and Quality System Gibco® AmnioMAX™ C-100 Complete Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. Contents & storage Basal Medium (90 ml): Storage: 2 – 8°C Ships: Ambient temperature Shelf life: 16 months from date of manufacture Supplement (15 ml): Storage: -5 to -20°C Ships: Frozen Shelf life: 16 months from date of manufactureUse complete medium within 7–10 days once opened.

12557021 - PB-MAX™ Karyotyping Medium

Gibco® PB-MAX™ Karyotyping Medium is a fully-supplemented medium developed for the short-term culture of peripheral blood lymphocytes for cytogenetic studies and in vitro diagnostic procedures. Gibco® PB-MAX™ Karyotyping Medium features: • Ready-to-use format • Quality and performance testing • Classic formulation Ready-to-use format Gibco® PB-MAX™ Karyotyping Medium is a frozen, 1X medium, ready to use upon thawing. No supplementation is required. Thawed medium can be stored at 2–8°C for up to 14 days. Quality and performance testing Every lot of Gibco® PB-MAX™ Karyotyping Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Peripheral blood lymphocytes from a normal adult donor are cultured for 72 hours in Gibco® PB-MAX™ Karyotyping Medium before measuring the mitotic index and chromosome banding resolution. In addition, each lot is tested for sterility, pH, osmolality, and mycoplasma. Classic formulation Gibco® PB-MAX™ Karyotyping Medium is an optimized RPMI 1640 medium, supplemented with Fetal Bovine Serum (FBS), L-glutamine, and phytohemagglutinin (PHA). Gibco® PB-MAX™ Karyotyping Medium is based on formulations referenced in the Association for Cytogenetic Technologists Laboratory Manual for the culture of peripheral blood lymphocytes for cytogenetic analysis. The complete formulation is available. Product Use For in vitro diagnostic use. cGMP Manufacturing and Quality System Gibco® PB-MAX™ Karyotyping Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. Contents & storage Storage conditions: -5 to -20°C. Protect from light. Shipping conditions: Frozen Shelf life: 12 months from date of manufacture

12556023 - AmnioMAX™ C-100 Supplement

Gibco® AmnioMAX™ C-100 Supplement is part of a kit used to prepare a fully-supplemented Gibco® AmnioMAX™ C-100 Complete Medium developed for the short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures. Gibco® AmnioMAX™ C-100 Complete Medium features: • Easy-to-use format • Quality and performance testing • Unique, optimized formulation Easy-to-use format Gibco® AmnioMAX™ C-100 Complete Medium is a two-part kit for preparation of a complete medium that requires no additional supplementation. The complete medium can be stored at 2–8°C for up to ten days. Quality and performance testing Every lot of Gibco® AmnioMAX™ C-100 Complete Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Pooled primary human amniotic fluid samples are cultured for six days in Gibco® AmnioMAX™ C-100 Complete Medium before measuring the total number of colonies and total number of mitotic colonies. In addition, each lot is tested for sterility, pH and osmolality. Unique, optimized formulation Gibco® AmnioMAX™ C-100 Complete Medium contains Fetal Bovine Serum (FBS), gentamicin, and L-glutamine to maximize cell attachment and growth. This optimized medium also has an enhanced buffering system that provides greater pH stability during culture manipulations. Product Use For in vitro diagnostic use. cGMP Manufacturing and Quality System Gibco® AmnioMAX™ C-100 Supplement is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. Contents & storage Storage conditions: -5 to -20°C Shipping conditions: Frozen Shelf life: 16 months from date of manufacture. Use complete medium within 7–10 days once opened.