- Target Molecule: Protein
- Label or Dye: SilverQuest™ Silver Stain
- Detection Method: Colorimetric
- Detection Location: In-Gel Detection
- Product Line: SilverQuest™
- Product Size: 1 kit
The SilverQuest™ Silver Staining Kit provides a rapid and easy method to silver stain proteins and DNA in polyacrylamide gels. Silver staining allows detection of most proteins since it is 30-fold more sensitive than staining with colloidal Coomassie® G-250. The SilverQuest™ Silver Staining Kit is specifically designed to provide sensitive silver staining compatible with mass spectrometry analysis. Features of this kit:
Mass spectrometry–compatible reagents
The SilverQuest™ Silver Staining Kit has been specifically designed to make protein detection compatible with mass spectrometry analysis. The SilverQuest™ reagent set contains a sensitizer that contains no glutaraldehyde or formaldehyde, so it leaves lysine and arginine side-chains unmodified. Additionally, the SilverQuest™ destaining solution effectively removes silver from protein bands in polyacrylamide gels. Peptides remain as efficient targets for in-gel trypsin digestion and gel extraction and yield better coverage during mass spectrometry analysis.
This kit contains sufficient reagents to stain 25 minigels
250 mL Sensitizer
25 mL Stainer
250 mL Developer
2 mL Developer Enhancer
250 mL Stopper solution
60 mL Destainer A
60 mL Destainer B
Store kit at room temperature (15°C to 30°C). Stable for 6 months.
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