Gel/PCRTM DNA Isolation System

Features and Benefits

  • Dual Gel/PCR system
  • Efficient extraction of DNA fragments from 100-bp to 10-kbp
  • High purity of DNA (A260/A280 1.8-1.9)
  • >95% primer and salts removal
  • Recover DNA fragments from standard of low-melting point agarose gels in TAE or TBE buffer
  • Elute DNA with just 15-30µl Elution buffer or ddH20
  • Recovery up to 90%
  • Preparation Time: 10-15 minutes
  • No sodium Iodide to interfere with subsequent reactions
  • No shearing of large DNA fragments
Product Cat.No. List price Contents MSDS Protocol
Gel/PCRTM DNA Isolation System GP1001 50 preps
50 GP Columns,
50 Collection Tubes,
Buffers, Protocol

GP1002 250 preps
250 GP Columns,
250 Collection Tubes,
Buffers, Protoco

Principle

Viogene dual Gel/PCRTM DNA Isolation System contains a silica-gel-membrane for binding of DNA in high-salt buffer (pH 4.5~5.5) and elution of DNA with low-salt buffer (pH 7.0~9.0) or ddH2O. The purification procedure removes primers, nucleotides, enzyme, salts, ethidium bromide and agarose impurities from DNA samples.

Downstream Application

Viogene Gel/PCRTM DNA Isolation System provides reproducible yields of high-purity DNA suitable for use in most applications, including:

  • Automated fluorescent and radioactive sequencing & PCR
  • Restriction digestion & modifying enzymatic reaction
  • Ligation
  • labeling, & hybridization