The Genomic DNA system uses the silica-gel-membrane technology for simple and fast isolation of Genomic DNA without phenol/chloroform or ethanol precipitation. Homogenization is not necessary since tissues are directly lysed by Proteinase K. The buffer system is optimized to allow selective binding of DNA to the silica-gel membrane. The simple centrifugation protocol completely removes contaminants such as proteins, divalent cations, and secondary metabolites. Pure DNA is then eluted in water or low-salt buffer, ready to use. Special protocols and guidelines are provided for different samples, such as tissues, culture cells, blood, mouse tails, bacteria and yeast.
STAR Prep. purified DNA is free from contaminants and enzyme inhibitors, and typically has A260/A280 ratios between 1.7 and 1.9. The DNA is sized up to 50-kb, with predominant fragments of 30-kb, and is suitable for applications such as:
- Restriction digestion
- Southern blotting
- PCR, Real-time PCR