The Genomic DNA system uses the silica-gel-membrane technology for simple and fast isolation of Genomic DNA without phenol/chloroform or ethanol precipitation. The buffer system is optimized to allow selective binding of DNA to the silica-gel-membrane. The simple centrifugation process completely removes contaminants such as proteins, divalent cations, and secondary metabolites. Pure DNA is then eluted in water or low-salt buffer and ready to be used.
Genomic DNA purified by Viogene Blood Genomic DNA Extraction System is free from contaminants and enzyme inhibitors, and typically has A260 / A280 ratios between 1.7 and 1.9. The DNA is sized up to 50-kb, with predominant fragments of 30-kb, and is suitable for applications such as:
- Restriction digestion
- Southern Blotting
- PCR, Real-time PCR