Geno Plus Genomic DNA Extraction Maxiprep System
Features and Benefits
- Rapid isolation (only 50-90 minutes) of ready-to-use genomic DNA from whole blood
- Pure DNA, free from contaminants and enzyme inhibitors
- Spin column fits centrifugation or vacuum manifold
- No organic extraction (without phenol/ chloroform) & no ethanol precipitation needed
| Product | Cat No. | List price | Contents | MSDS | Protocol |
|---|---|---|---|---|---|
| Geno Plus Genomic DNA Extraction Maxiprep System | GGM2001 | 10 preps | 10 Genomic Maxi Columns, 10 Collection Tubes, Buffers, Protocol, Proteinase K |  | |
| GGM2002 | 25 preps | 25 Genomic Maxi Columns, 25 Collection Tubes, Buffers, Protocol, Proteinase K | | |
Principle
The Genomic DNA system uses the silica-gel-membrane technology for simple and fast isolation of Genomic DNA without phenol/chloroform or ethanol precipitation. The buffer system is optimized to allow selective binding of DNA to the silica-gel-membrane. The simple centrifugation process completely removes contaminants such as proteins, divalent cations, and secondary metabolites. Pure DNA is then eluted in water or low-salt buffer and ready to be used.
Downstream Applications
Genomic DNA purified by Viogene Blood Genomic DNA Extraction System is free from contaminants and enzyme inhibitors, and typically has A260 / A280 ratios between 1.7 and 1.9. The DNA is sized up to 50-kb, with predominant fragments of 30-kb, and is suitable for applications such as:
- Restriction digestion
- Southern Blotting
- RAPD
- RFLP
- PCR, Real-time PCR