The Viogene Plant Genomic DNA Extraction System uses silica-gel membrane technology for simple and fast isolation of Genomic DNA without phenol/chloroform or ethanol precipitation. Homogenization is not necessary since tissues can be directly lysed by Proteinase K and filtered by shearing tube.
Genomic DNA purified by Viogene system is free from contaminants and enzyme inhibitors, and typically has A260 / A280 ratios between 1.7 and 1.9. The DNA is sized up to 50-kb, with predominant fragments of 30-kb, and is suitable for applications such as:
- Restriction digestion
- Southern blotting
- RAPD, RFLP
- PCR, Real-time PCR