Mini Plus Plasmid DNA Extraction System
Features and Benefits
- 20-30 minutes binding-washing-elution protocol
- Optional for spin or vacuum format
- No organic solvents (phenol/chloroform) extraction needed
- Reproducible yields of high-purity plasmid DNA
| Product | Cat No. | Kit Size | Contents | MSDS | Protocol |
|---|---|---|---|---|---|
| Mini Plus Plasmid DNA Extraction System | GF2001 | 50 Preps | 50 Mini Plus Column, 50 Collection Tubes, Buffers, RNase A, Protocol |  | |
| GF2002 | 250 Preps | 250 Mini Plus Column, 250 Collection Tubes, Buffers, RNase A, Protocol | | |
Principle
Contains a unique silica-gel-membrane which binds up to 40 µg DNA in the presence of high concentration of chaotropic salt, and allows elution in a small volume of low salt buffer. Viogene membrane technology eliminates time consuming phenol/chloroform extraction and alcohol precipitation, as well as the problems and inconvenience associated with loose resins and slurries. High-purity plasmid DNA eluted from Viogene Mini PlusTMmodules is immediately ready to use, no need to precipitate, concentrate, or desalt. Viogene Mini PlusTM spin columns can be used either in microcentrifuges or on vacuum manifolds. The vacuum procedure enables simplified handling and faster sample processing.
Downstream Applications
Viogene Mini PlusTMsystem provides reproducible yields of high-purity DNA suitable for using in most applications, including:
- Restriction digestion
- Transformation
- PCR
- Automated fluorescent and radioactive sequencing
- Library screening or Large-scale