Principle
Viogene Total RNA Extraction Maxiprep System provides an economical method to purify total RNA from various samples such as cultured cells, tissues, and bacteria. A simple silica-membrane spin-column method can isolate total RNA without need of performing time-consuming phenol/ chloroform extraction and ethanol precipitation. Total RNA longer than 200 nucleotides are isolated, while small RNA such as 5.8S RNA, 5S RNA, and tRNA, which make up 15-20% of the total RNA, are excluded.NA, 5S RNA, and tRNA, which make up 15-20% of the total RNA, are excluded.
Downstream Applications
- Northern blotting
- Ploy A+ RNA selection
- cDNA synthesis
- RT-PCR