Gel/PCRTM DNA Isolation System
Features and Benefits
- Dual Gel/PCR system
- Efficient extraction of DNA fragments from 100-bp to 10-kbp
- High purity of DNA (A260/A280 1.8-1.9)
- >95% primer and salts removal
- Recover DNA fragments from standard of low-melting point agarose gels in TAE or TBE buffer
- Elute DNA with just 15-30µl Elution buffer or ddH20
- Recovery up to 90%
- Preparation Time: 10-15 minutes
- No sodium Iodide to interfere with subsequent reactions
- No shearing of large DNA fragments
Principle
Viogene dual Gel/PCRTM DNA Isolation System contains a silica-gel-membrane for binding of DNA in high-salt buffer (pH 4.5~5.5) and elution of DNA with low-salt buffer (pH 7.0~9.0) or ddH2O. The purification procedure removes primers, nucleotides, enzyme, salts, ethidium bromide and agarose impurities from DNA samples.
Downstream Application
Viogene Gel/PCRTM DNA Isolation System provides reproducible yields of high-purity DNA suitable for use in most applications, including:
- Automated fluorescent and radioactive sequencing & PCR
- Restriction digestion & modifying enzymatic reaction
- Ligation
- labeling, & hybridization