PCR AdvancedTM PCR Clean Up System
Features and Benefits
- Clean up and concentrate DNA in 5 minutes, such as OrageneTM DNA
- Up to 95% recovery of DNA fragment (100bp to >23kb)
- High purity of DNA (A260 /A280 >1.9)
- > 95% contaminant removal
- Elute DNA with just 15~30 µl Elution buffer or ddH2O
Principle
PCR AdvancedTM PCR Clean Up Miniprep System contains a silica-gel-membrane for binding of DNA in high-salt buffer (pH 4.5~5.5) and elution of DNA with low-salt buffer (pH 7.0~9.0) or ddH2O. The purification procedure removes contaminants, salts and other impurities from DNA samples,such as OrageneTM DNA.
Downstream Application
PCR AdvancedTM PCR Clean Up Miniprep System provides reproducible yields of high-purity DNA suitable for use in most applications, including:
- Automated sequencing & PCR
- Restriction digestion & modifying enzymatic reaction
- Ligation
- labeling, & hybridization