Endotoxin-Free Utrapure Plasmid Extraction Midiprep System
Features and Benefits
- Rapid and simple extraction
- Reproducible high yields of ultrapure DNA
- No toxic reagent such as ethidium bromide, phenol/chloroform, CsCl involved
- Effective endotoxin removal step
- Endotoxin level can be consistently less than 0.1 EU/µg DNA in the final DNA product
| Product | Cat.No. | List price | Contents | MSDS | Protocol |
|---|---|---|---|---|---|
| Endotoxin-Free Utrapure Plasmid Extraction Midiprep System | GDN1001 | 10preps | 10 Maxi Plus Column, Buffers, RNase A, Protocol | N/A |
|
Principle
Endotoxin-Free Utrapure Plasmid Extraction Midiprep System is composed of macroporous silica–based resin with a high density of diethylaminoethyl (DEAE) group, which is developed exclusively for isolation of nucleic acids.Purification is based on the interaction between negatively charged phosphates of nucleic acid backbones and positively charged DEAE groups on the surface of the resin.
An innovative endotoxin-removal reagent, E2 reagent, can effectively remove lipopolysaccharides from bacterial cell lys.