Low-Endotoxin Plasmid DNA Maxiprep Extraction System
| Product | Cat.No. | Kit Size | Contents | MSDS | Protocol |
|---|---|---|---|---|---|
| Low-Endotoxin Plasmid DNA Maxiprep Extraction System | GFM1001 | 10preps | 10 Maxi Plus Column, Buffers, RNase A, Protocol | N/A |
|
| GFM1002 | 25preps | 25 Maxi Plus Column, Buffers, RNase A, Protocol | N/A |
|
Principle
Viogene Low-Endotoxin Plasmid DNA Maxiprep Extraction System provides a simple, fast and cost-effective method to purify plasmid DNA without phenol/chloroform extraction. It is based on binding of DNA to silica-based membranes in chaotropic salts. An average yield of 500 mg of plasmid DNA can be expected from 100 ml overnight bacterial culture.
Downstream Applications
- Restriction digestion
- Radioactive and fluorescent sequencing
- Transformation / Transfection
- Ligation
- PCR, RAPD
