Total RNA Extraction Midiprep System

Features and Benefits

  • Purification process requires only 20-30 minutes
  • Spin column fit by centrifugation
  • No toxic reagent (no phenol/chloroform, no CsCl gradients, no LiCl precipitation)
  • Ready-to-use high-quality RNA (Purity A260 /A280 >1.9) from small to large amounts of starting material


ProductCat No.List priceContentsMSDS Protocol
Total RNA Extraction Midiprep SystemGRD100110 preps
10 RNA Midi Columns,
Buffers, Protocol,
RNase-free ddH2O
GRD100250 preps
50 RNA Midi Columns,
Buffers, Protocol,
RNase-free ddH2O


The system uses reagents quickly permeating the biological samples and thus simplifies total RNA extraction in combination of the stringency of guanidine-thiocyanate lysis and silica-gel-membrane purification. This system has no need of phenol/chloroform extraction and centrifugation with CsCl gradients or precipitation with LiCl or ethanol.

Downstream Applications

RNA purified by Viogene Total RNA Extraction System has A260/A280 ratio of 1.9~2.1, and is ideal for all applications including:

  • Northern, dot and slot blotting
  • RT-PCR / Quantitative real-time PCR
  • Poly A+ RNA selection
  • cDNA Synthesis/ Primer extension
  • Array analysis
  • In vitro translation