Total RNA Extraction Midiprep System
Features and Benefits
- Purification process requires only 20-30 minutes
- Spin column fit by centrifugation
- No toxic reagent (no phenol/chloroform, no CsCl gradients, no LiCl precipitation)
- Ready-to-use high-quality RNA (Purity A260 /A280 >1.9) from small to large amounts of starting material
Principle
The system uses reagents quickly permeating the biological samples and thus simplifies total RNA extraction in combination of the stringency of guanidine-thiocyanate lysis and silica-gel-membrane purification. This system has no need of phenol/chloroform extraction and centrifugation with CsCl gradients or precipitation with LiCl or ethanol.
Downstream Applications
RNA purified by Viogene Total RNA Extraction System has A260/A280 ratio of 1.9~2.1, and is ideal for all applications including:
- Northern, dot and slot blotting
- RT-PCR / Quantitative real-time PCR
- Poly A+ RNA selection
- cDNA Synthesis/ Primer extension
- Array analysis
- In vitro translation