Plant Total RNA Extraction Miniprep System

Features and Benefits

  • Purification process requires only 20-30 minutes
  • Spin column fit by centrifugation
  • No toxic reagent (no phenol/chloroform, no CsCl gradients, no LiCl precipitation)
  • Ready-to-use high-quality RNA (Purity A260 /A280 >1.9) from small to large amounts of starting material

 

ProductCat No.List priceContentsMSDS Protocol
Total RNA Extraction Miniprep SystemGR100150 preps
50 RNA Mini Columns,
50 Collection Tubes,
50 Elution Tubes,
Buffers, Protocol,
Rnase-free ddH2O
GR1002250 preps
250 RNA Mini Columns,
250 Collection Tubes,
250 Elution Tubes,
Buffers, Protocol,
RNase-free ddH2O

Principle

The system uses reagents quickly permeating the biological samples and thus simplifies total RNA extraction in combination of the stringency of guanidine-thiocyanate lysis and silica-gel-membrane purification. This system has no need of phenol/chloroform extraction and centrifugation with CsCl gradients or precipitation with LiCl or ethanol.

Downstream Applications

RNA purified by Viogene Total RNA Extraction System has A260/A280 ratio of 1.9~2.1, and is ideal for all applications including:

  • Northern, dot and slot blotting
  • RT-PCR / Quantitative real-time PCR
  • Poly A+ RNA selection
  • cDNA Synthesis/ Primer extension
  • Array analysis
  • In vitro translation