Viral RNA Extraction Miniprep System
Features and Benefits
- Viral RNA purification process requires only 20-30 minutes
- Spin column fit by centrifugation
- No toxic reagent (no phenol/chloroform, no CsCl gradients, no LiCl precipitation)
- Ready-to-use high-quality RNA (Purity A260 /A280 >1.9) from small to large amounts of starting material
Principle
The system uses reagents for fast purification of biological fluids containing RNA virus, simplifies viral RNA extraction in combination of the stringency of guanidine-thiocyanate lysis and silica-gel-membrane purification. This system does not need of phenol/chloroform extraction and centrifugation with CsCl gradients or precipitation with LiCl or ethanol.
Downstream Applications
RNA purified by Viogene Viral RNA Extraction System has A260/A280 ratio of 1.9~2.1, and is ideal for all applications including:
- Northern, dot and slot blotting
- RT-PCR / Quantitative real-time PCR
- Poly A+ RNA selection
- cDNA Synthesis/ Primer extension
- Array analysis
- In vitro translation